Peptide mapping

Enzymatic digest and LC-MS/MS to confirm primary structure and locate modifications on peptides and proteins.

At a glance

Turnaround

Quoted with the project.

Price

Quoted with the project.

Sample

Confirm with the laboratory when ordering.

Accreditation

ISO 17025 quality system, scope extension in progress

Scope

Sequence coverage of the digested peptide or protein.
Site assignment of oxidation, deamidation, disulfide pairing and other modifications.
Lot-to-lot comparison of the map.

Method

Digest with trypsin or a protease suited to the sequence; reduction and alkylation where disulfides are present.
LC-MS/MS on the Orbitrap; peptides matched to the expected sequence.

Reported as

Coverage map, peptide table with observed masses, modification sites, base peak chromatogram.

Sample

Confirm with the laboratory when ordering.

Accreditation

Performed under the laboratory’s ISO/IEC 17025:2017 quality system. A2LA scope extension (certificate 6377.01) in progress.

Order

Start an order online, or send the compound name and the tests you need for a quote.

All peptide testing