Phenols in three Echinacea species, hypericin, silymarins and valerenic acids.
| Tests | Total phenols in Echinacea purpurea, E. angustifolia and E. pallida; hypericin; silymarins; valerenic acids; hyperforin; total silybins |
|---|---|
| Method | HPLC or UPLC; UV/Vis for the hypericin and silymarins estimates |
| Results | Reported against your specification or label claim, with pass/fail where limits apply. |
| Price | Quoted per project |
The chromatographic tests separate the named compounds and measure them against reference standards. The UV/Vis tests are estimates of a compound group, described in their sections.
The three Echinacea species have different phenolic profiles, so each has its own test.
Measures phenolic compounds in Echinacea purpurea by HPLC and reports their total. The USP Echinacea purpurea Root monograph calculates total phenols as the sum of caftaric acid, chicoric acid and chlorogenic acid. Tell us whether the material is root or aerial parts.
Measures phenolic compounds in Echinacea angustifolia by HPLC and reports their total. The USP Echinacea angustifolia monograph calculates total phenols as the sum of echinacoside, dicaffeoylquinic acid and chlorogenic acid, a different set from E. purpurea.
Measures phenolic compounds in Echinacea pallida by HPLC and reports their total. USP names E. pallida root as a source in its Echinacea species capsule and tablet monographs, which calculate total phenols for the dry extracts as the sum of caftaric acid, chicoric acid, echinacoside and cynarin. Tell us the specification you report against.
Measures hypericin in St. John's wort materials by HPLC. USP describes St. John's wort flowering top as the dried flowering tops of Hypericum perforatum and sets requirements for hyperforin and for the combined total of hypericin and pseudohypericin; tell us whether your specification includes pseudohypericin.
A UV/Vis estimate of hypericins in St. John's wort, expressed as hypericin. The result reflects the combined absorbance of the prepared sample solution, so other compounds that absorb in the same region can affect it; it does not identify individual compounds. Use the HPLC test for a compound-specific result.
Measures the silymarin components of milk thistle by UPLC. USP describes milk thistle as the dried ripe fruit of Silybum marianum and, for capsules, calculates silymarin as silybin from the sum of silydianin, silychristin, silybin A, silybin B, isosilybin A and isosilybin B.
A UV/Vis estimate of silymarin in milk thistle, expressed as a single reference compound. The result reflects the combined absorbance of the prepared sample solution, so other compounds that absorb in the same region can affect it; it does not identify individual compounds. Use the UPLC test for individual components.
Measures valerenic acids in valerian by HPLC. USP describes valerian as the subterranean parts of Valeriana officinalis and sets requirements for valerenic acid and for total valerenic acids, calculated as the sum of hydroxyvalerenic acid, acetoxyvalerenic acid and valerenic acid.
Measures hyperforin, a phloroglucinol derivative of St. John's wort (Hypericum perforatum), by HPLC as a single compound.
Measures silybin A and silybin B, flavonolignans of milk thistle, by UPLC and reports them individually or as a total, as your specification states.
Send the material sealed, labeled and protected from light.
Tell us the material, the plant part or extract, and the specification or label claim. We confirm the sample amount at quote.
Questions about a sample or a method, call 360-967-7010 or email [email protected].
