Assays for aloins, cascara sagrada hydroxyanthracene derivatives and sennoside-type glycosides.
| Tests | Aloin A, aloin B, aloin A+B, cascara sagrada hydroxyanthracene derivatives and cascarosides, hydroxyanthracene glycosides as sennoside B |
|---|---|
| Method | HPLC for the aloins and for hydroxyanthracene glycosides (expressed as sennoside B); UV/Vis for cascara sagrada |
| Results | Reported against your specification or label claim, with pass/fail where limits apply. |
| Price | Quoted per project |
Aloe, cascara sagrada and senna contain hydroxyanthracene compounds. The HPLC assays separate the named compounds and measure them against reference standards. The cascara sagrada assays are UV/Vis estimates of a compound group, expressed as a single reference compound.
Specifications for these materials name the compound or group and the reference compound a result is expressed as. Tell us which specification applies so the result is reported on the same terms.
Measures aloin A in aloe materials and products by HPLC against a reference standard. Aloin A and aloin B are closely related isomers; this entry reports aloin A only. The cited USP Aloe monograph preview (2022, DOI 10.31003/USPNF_M1540_05_01) defines aloe as the dried latex of the leaves of Aloe vera or Cape aloe and states its minimum content as aloin A.
Measures aloin B in aloe materials and products by HPLC against a reference standard, reported separately from aloin A.
Measures aloin A and aloin B by HPLC and reports their sum. Tell us whether your specification states aloin as the sum, as aloin A alone or as each isomer.
Cascara sagrada is the dried bark of Frangula purshiana. This UV/Vis assay estimates its total hydroxyanthracene derivatives. The result reflects the combined absorbance of the prepared sample solution, so other compounds that absorb in the same region can affect it. It is not an identification of individual compounds. The USP cascara sagrada monographs calculate this total as cascaroside A; tell us which specification applies.
Estimates both the total hydroxyanthracene derivatives and the cascaroside portion of cascara sagrada bark or extract by UV/Vis. The result reflects the combined absorbance of the prepared sample solution, so other compounds that absorb in the same region can affect it. It is not an identification of individual compounds. The USP cascara sagrada monographs calculate both values as cascaroside A and set a minimum share of cascarosides within the total.
Measures hydroxyanthracene glycosides by HPLC and expresses the total as sennoside B. Sennosides are the hydroxyanthracene glycosides of senna; the USP Senna Leaf monograph calculates its anthraquinone glucosides as sennosides, so tell us the material and the specification you report against.
Send the material sealed, labeled and protected from light.
Tell us the material, the label claim or specification and the basis it is stated on. We confirm the sample amount at quote.
Questions about a sample or a method, call 360-967-7010 or email [email protected].
