Methylene blue

Methylene blue: small molecule (phenothiazinium dye).

Purity and quantity by the chromatographic method matched to the compound (HPLC-UV, LC-MS or GC-MS), with identity against a reference standard. $250 per sample; raw powder purity $200. Results in 3 to 5 business days.

Full USP monograph testing is available. See USP Testing.

Structure

2D structure of methylene-blue drawn in Vanguard Laboratory colors

Methylene blue: 2D structure drawn from the PubChem record. Stereochemistry shown as registered.

Compound data

CAS number

61-73-4 (anhydrous)

Formula

C16H18ClN3S (trihydrate C16H18ClN3S·3H2O)

Molecular weight

319.9 g/mol (anhydrous); 373.9 (trihydrate)

Type

small molecule (phenothiazinium dye)

Structure and names

IUPAC name or sequence: [7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium chloride

Other names: Methylthioninium chloride, basic blue 9; trihydrate CAS 7220-79-3

Anhydrous and trihydrate differ by 54 g/mol; state which form the label claim refers to.

Tests and prices

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on testPrice
Identity and impurities
FTIR identity$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)$125
Chiral purity (where a chiral method exists; new methods quoted)$250
Water, solvents and salt form
Water content by Karl Fischer$60
Residual solvents$150
Counterions: chloride, acetate and TFA$70
Assay
Assay, % w/w (with raw powder purity; $250 together)+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead$150
Conformity: purity and quantity on more units from the same lot$100 per extra unit
Microbiology (injectables)
Endotoxin$210
Sterility or bioburden$150
Container closure integrity (CCI)$50
Screening
Fentanyl screen$50

10% off for 10 or more samples.

Need something else? We run other tests on request.

Order

Start an order online, or send the compound name and the tests you need for a quote.