NAD+ is the oxidized form of nicotinamide adenine dinucleotide, a dinucleotide of nicotinamide riboside and adenosine joined by a pyrophosphate bridge[1, 2]. It occurs in all living cells.
NAD+ testing at Vanguard Laboratory: purity, quantity and identity from $250 per sample. Results in 3 to 5 business days; sterility 14 days.
On this page: Testing · Compound data · Structure and names · Research · Analytical notes · USP–NF · References
Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.
| Add-on test | Price |
|---|---|
| Identity and impurities | |
| FTIR identity | $90 |
| CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur) | $125 |
| Chiral purity (where a chiral method exists; new methods quoted) | $250 |
| Water, solvents and salt form | |
| Water content by Karl Fischer | $60 |
| Residual solvents | $150 |
| Counterions: chloride, acetate and TFA | $70 |
| Assay | |
| Assay, % w/w (with raw powder purity; $250 together) | +$50 |
| Metals and conformity | |
| Heavy metals: arsenic, cadmium, chromium, mercury and lead | $150 |
| Conformity: purity and quantity on more units from the same lot | $100 per extra unit |
| Microbiology (injectables) | |
| Endotoxin | $210 |
| Sterility or bioburden | $150 |
| Container closure integrity (CCI) | $50 |
| Screening | |
| Fentanyl screen | $50 |
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| Test | Purpose for this compound | Reported as | Sample | Turnaround |
|---|---|---|---|---|
| Purity and quantity | Quantifies NAD+. Other peaks, such as NADH, nicotinamide or ADP-ribose, are reported as detected; identifying them is a separate request. | Purity, area percent, two decimals. Quantity, mg per unit, with label claim. | 1 sample unit; contact the laboratory about quantity for the submitted format. | 3 to 5 business days from receipt. |
| Identity | Routine identity: retention time and UV spectrum against a reference standard. It does not establish stereochemistry. | Match or no match, with the basis stated. | No additional sample unit. | 3 to 5 business days, with purity and quantity. |
| LC-MS identity | Mass evidence for NAD+ (663.11 Da) versus NADH (665.12 Da), NMN or NR. Quoted separately. | Observed mass, expected mass, mass spectrum. | Confirm sample quantity and the compound list before shipping. | 3 to 5 business days from receipt. |
| Water content | Hygroscopic and often a hydrate; water changes content on an as-is basis. | Water content, percent w/w. | Sample quantity depends on the requested tests; contact the laboratory before shipping. | 3 to 5 business days from receipt. |
| Counterion | Salt form (free acid or sodium salt) changes NAD+ content per gram. Sodium is measured by ion chromatography, quoted per project. | Counterion content, percent w/w. | Sample quantity depends on the requested tests; contact the laboratory before shipping. | 3 to 5 business days from receipt. |
| Endotoxin | Relevant for injectable or solution preparations. | EU per vial, numeric, or below the reporting limit. Reporting limit, dilution and the applicable limit. | Sample quantity depends on the requested tests; contact the laboratory before shipping. | 3 to 5 business days from receipt. |
What these results do not show: results describe the sample tested. Purity and content do not establish biological activity, sterility, or the absence of contaminants that were not tested.
NAD+: 2D structure drawn from the PubChem record. Stereochemistry shown as registered.
dinucleotide coenzyme (pyridine nucleotide)
C21H27N7O14P2
663.4 g/mol (NAD+ inner salt / ‘free acid’ form); sodium salt C21H26N7NaO14P2 685.4 g/mol
Monoisotopic 663.1091 Da
53-84-9
Modifications:
Stereochemistry: beta-D-ribofuranosyl units, (2R,3S,4R,5R) at both riboses
CAS note: 53-84-9 is oxidized NAD+ as the inner salt (zwitterion), sold as ‘free acid’. Sodium salt is 20111-18-6. Reduced NADH is 58-68-4 (C21H29N7O14P2, 665.4) and NADH disodium salt is 606-68-8 (709.4). Commercial lots may be hydrates.
Other names: nadide (INN, USAN), nicotinamide adenine dinucleotide, beta-NAD, NAD, diphosphopyridine nucleotide (DPN), coenzyme I, beta-NAD sodium salt (salt form)
PubChem CID 5892 · UNII 0U46U6E8UK[1, 3, 4]
Structural features:
FDA lists NAD and NADH disodium in 503A bulk substance Category 1 (under evaluation) as of May 14, 2026[5].
Summaries of published research, for reference only. Not a claim of safety or efficacy.
No official USP–NF monograph for NAD+ was found.
Related general chapters: 〈921〉 Water Determination, 〈85〉 Bacterial Endotoxins Test, 〈71〉 Sterility Tests. General chapters describe test methods; they are not compound-specific monographs. Chapters numbered 1000 to 1999, such as 〈1503〉, are informational.
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