Anthocyanin, Proanthocyanidin and Polyphenol Assays

Anthocyanins, proanthocyanidins, procyanidins, punicalagins, ellagic acid, tannins and total polyphenols in fruit, seed, bark and tea materials and branded ingredients.

Data sheet

TestsBilberry, elderberry, black rice, Delphinol and Morosil anthocyanins; grape seed, maritime pine, pine bark, cranberry, tart cherry and hawthorn proanthocyanidins or procyanidins; total polyphenols as gallic acid; tea polyphenols as ethyl gallate; total polyphenols in Fitnox; punicalagins; ellagic acid; hydrolysable tannins in Capros; theobromine
MethodHPLC or UPLC; UV/Vis or Folin-Ciocalteu for the estimates
ResultsReported against your specification or label claim, with pass/fail where limits apply.
PriceQuoted per project

How it works

The HPLC and UPLC content assays separate the named compounds and measure them against reference standards. The maritime pine identification compares the chromatographic profile of the sample with that of a reference and gives an identification, not a content value. The UV/Vis and Folin-Ciocalteu tests are estimates of a compound group, described in their sections.

Proanthocyanidin and polyphenol results depend on the method and the reference compound, so results from different methods are not interchangeable. Tell us the method your specification uses.

Tests offered

Anthocyanidins and anthocyanosides content in bilberry by HPLC

Measures anthocyanosides (anthocyanin glycosides) and anthocyanidins (their aglycones) in bilberry by HPLC. The USP Powdered Bilberry Extract monograph describes that extract as prepared from the ripe fruits of Vaccinium myrtillus and calculates anthocyanosides as cyanidin-3-O-glucoside chloride and anthocyanidins as cyanidin chloride.

Anthocyanosides and cyanidin content in elderberry by HPLC

Measures anthocyanosides and cyanidin in elderberry materials by HPLC. Tell us the species, the plant part and the specification you report against.

Oligomeric proanthocyanidins content in grape seed by HPLC

Measures oligomeric proanthocyanidins in grape seed extract by HPLC. USP describes Grape Seeds Oligomeric Proanthocyanidins as a fraction of an extract of the ripe seeds of Vitis vinifera and states its content on the anhydrous basis.

Maritime pine extract identification by HPLC

An identity test for maritime pine extract that compares the HPLC profile of the sample with that of a reference. USP describes maritime pine as the bark of the stems of Pinus pinaster and lists a Maritime Pine Extract reference standard. The result is an identification, not a content value.

Procyanidins content in maritime pine by UV/Vis

A UV/Vis estimate of procyanidins in maritime pine bark or extract. The result reflects the combined response of the prepared sample solution, so other compounds that respond in the same way can affect it; it does not identify individual compounds. The USP Maritime Pine and Maritime Pine Extract monographs state procyanidins on the dried basis; tell us which material and specification apply.

Proanthocyanidins content in pine bark by UV/Vis

A UV/Vis estimate of proanthocyanidins in pine bark materials. The result reflects the combined response of the prepared sample solution, so other compounds that respond in the same way can affect it; it does not identify individual compounds. Tell us the species and the specification you report against.

Proanthocyanidins content in cranberry by UV/Vis

A UV/Vis estimate of proanthocyanidins in cranberry materials. The result reflects the combined response of the prepared sample solution, so other compounds that respond in the same way can affect it; it does not identify individual compounds. Proanthocyanidin results depend strongly on the method and the reference used, so tell us the specification you report against.

Proanthocyanidins content in tart cherry by UV/Vis

A UV/Vis estimate of proanthocyanidins in tart cherry materials. The result reflects the combined response of the prepared sample solution, so other compounds that respond in the same way can affect it; it does not identify individual compounds.

Procyanidins expressed as cyanidin chloride in hawthorn berries by UV/Vis

A UV/Vis estimate of procyanidins in hawthorn berries, expressed as cyanidin chloride. The result reflects the combined response of the prepared sample solution, so other compounds that respond in the same way can affect it; it does not identify individual compounds.

Total polyphenols as gallic acid (Folin-Ciocalteu) by UV/Vis

A colorimetric estimate of total polyphenols using the Folin-Ciocalteu reagent, expressed as gallic acid. The reagent reacts with phenolic compounds and with other reducing substances such as ascorbic acid, so the result is not specific to any one compound class and does not identify individual compounds.

Punicalagins A+B content by HPLC

Measures punicalagin A and punicalagin B by HPLC and reports their sum. Tell us whether your specification states the sum or each form.

Ellagic acid content by HPLC

Measures ellagic acid by HPLC as a single compound. Free ellagic acid is a different result from ellagic acid released from ellagitannins; tell us which your specification means.

Ellagic acid, punicalagins and theobromine content by HPLC

Measures ellagic acid, punicalagins and theobromine together by HPLC, each reported against your specification.

Total anthocyanins in black rice extract by HPLC

Measures anthocyanins in black rice extract by HPLC and reports a total. Tell us which anthocyanins the total includes and the compound it is expressed as.

Anthocyanins in Delphinol by HPLC

Measures anthocyanins in Delphinol ingredient material by HPLC. Tell us which anthocyanins your specification names.

Anthocyanins in Morosil by UV/Vis

Determines anthocyanins in Morosil ingredient material by UV/Vis, expressed as your specification states. The exact procedure is confirmed at quote.

Tea polyphenols as ethyl gallate by UV/Vis

A UV/Vis group result for tea polyphenols, expressed as ethyl gallate. It does not identify individual catechins; for catechins by chromatography, see the tea assays.

Total polyphenols in Fitnox by UV/Vis

A UV/Vis group result for total polyphenols in Fitnox ingredient material, expressed as the reference compound your specification names.

Hydrolysable tannins in Capros by UPLC

Measures hydrolysable tannins in Capros ingredient material by UPLC. Tell us which compounds your specification names and whether it states each or a total.

Sending samples

Send the material sealed, labeled and protected from light and air.

Tell us the material, the plant part or extract, and the specification or label claim. We confirm the sample amount at quote.

Questions

Questions about a sample or a method, call 360-967-7010 or email [email protected].