Saponin and Glycoside Assays

Bacosides, gymnemic acids, Centella triterpenes, hederacoside C, mogrosides and steroidal saponins.

Data sheet

TestsBacosides, gymnemic acids, Centella asiatica triterpene derivatives, hederacoside C, mogrosides, protodioscin, diosgenin, tribulus, fenugreek and shatavari saponins, shatavarin IV, jujubosides; saponins in Fitnox and Astragin
MethodHPLC, UPLC or LC-MS; UV/Vis or gravimetry for the group estimates
ResultsReported against your specification or label claim, with pass/fail where limits apply.
PriceQuoted per project

How it works

The chromatographic tests separate the named compounds and measure them against reference standards. The UV/Vis and gravimetric tests are group estimates, described in their sections.

Saponin results from chromatographic, UV/Vis and gravimetric methods are not interchangeable, so tell us the method your specification uses.

Tests offered

Bacosides content by HPLC

Measures bacosides in bacopa materials by HPLC. USP describes bacopa as the dried stems and leaves of Bacopa monnieri and calculates its triterpene glycosides as the sum of bacopaside I, bacoside A3, bacopaside II, the jujubogenin isomer of bacopasaponin C and bacopasaponin C. Tell us which bacosides your specification names.

Bacosides content by UV/Vis

A UV/Vis estimate of bacosides in bacopa materials, expressed as a reference compound. The result reflects the combined response of the prepared sample solution and does not identify individual saponins; use the HPLC test for a compound-specific result.

Gymnemic acids content by HPLC

Measures gymnemic acids in gymnema materials by HPLC. USP describes gymnema as the dried leaves of Gymnema sylvestre and calculates gymnemic acids as gymnemagenin on the dried basis, with separate monographs for the powder and for native and purified extracts.

Gymnemic acids by gravimetry

A gravimetric estimate of gymnemic acids: the fraction isolated by the procedure is weighed. It is a group result defined by the procedure and is not interchangeable with an HPLC result calculated as gymnemagenin.

Triterpene derivatives content in Centella asiatica by HPLC

Measures Centella asiatica triterpene derivatives by HPLC. The USP Centella asiatica Triterpenes monograph, for an enriched fraction, calculates triterpene derivatives on the anhydrous basis as the sum of two or more of madecassoside, asiaticoside B, asiaticoside, madecassic acid, terminolic acid and asiatic acid. The USP Powdered Centella asiatica Extract monograph states them against the labeled amount, calculated on the dried basis as the sum of all six. Tell us which material and specification apply.

Hederacoside C content by HPLC

Measures hederacoside C, a triterpene saponin found in ivy leaf, by HPLC as a single compound.

Mogroside by HPLC

Measures mogrosides, the cucurbitane glycosides of monk fruit, by HPLC. Tell us whether your specification names mogroside V or a total.

Protodioscin content by UPLC

Measures protodioscin, a steroidal saponin, by UPLC as a single compound.

Diosgenin content by HPLC

Measures diosgenin, a steroidal sapogenin, by HPLC as a single compound. Free diosgenin and diosgenin released from saponins are different results; tell us which your specification means.

Saponins content in tribulus by UV/Vis

A UV/Vis estimate of saponins in Tribulus terrestris materials, expressed as a reference compound. It does not identify individual saponins.

Saponins content in fenugreek seed extract by gravimetry

A gravimetric estimate of saponins in fenugreek seed extract: the fraction isolated by the procedure is weighed. It is a group result defined by the procedure.

Total saponins in shatavari extracts by gravimetry

A gravimetric estimate of total saponins in shatavari (Asparagus racemosus) extracts: the fraction isolated by the procedure is weighed. It is a group result defined by the procedure.

Shatavarin IV content by LC-MS

Measures shatavarin IV, a steroidal saponin of shatavari, by liquid chromatography with mass spectrometric detection as a single compound.

Jujuboside content by UV/Vis

A UV/Vis estimate of jujubosides, the saponins of jujube seed, expressed as a reference compound. It does not identify individual jujubosides.

Saponins in Fitnox by gravimetry

A gravimetric result for saponins in Fitnox ingredient material: the saponin fraction is isolated as the procedure sets and weighed.

Saponins in Astragin by UV/Vis

Determines saponins in Astragin ingredient material by UV/Vis, expressed as your specification states. The exact procedure is confirmed at quote.

Sending samples

Send the material sealed, labeled and protected from light.

Tell us the material or extract and the specification or label claim. We confirm the sample amount at quote.

Questions

Questions about a sample or a method, call 360-967-7010 or email [email protected].