Vitamin D3 (cholecalciferol)

Vitamin D3: vitamin (secosteroid).

Vitamin D3 testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Vitamin D3: 2D structure drawn from the PubChem record (CID 5280795).

Compound data

CAS number

67-97-0

Formula

C₂₇H₄₄O

Molecular weight

384.6 g/mol (monoisotopic 384.34)

Type

vitamin (secosteroid)

Structure and names

IUPAC name: (1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-7a-methyl-1-[(2R)-6-methylheptan-2-yl]-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexan-1-ol

Other names: cholecalciferol

How Vanguard tests Vitamin D3

Identity, purity and quantity are measured by reversed-phase HPLC with UV detection against a reference standard, $250 per sample with results in 3 to 5 business days. Finished products use the same approach, with sample preparation matched to the product.

Full testing to the USP Cholecalciferol monograph is available and quoted: identification by infrared absorption, UV absorption and HPLC, and assay by HPLC with UV detection.

What's reported

Purity, as percent of total peak area
Quantity, in mg per serving or % w/w against the label
Identity, reported as match or no match against the reference standard

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

A USP monograph exists for Cholecalciferol. Monograph testing is quoted.
Tell us the salt or form the label states, so quantity is reported on the same basis.
One page covers Vitamin D3 and its salts and forms.

Questions about Vitamin D3 testing

Can you tell vitamin D3 from vitamin D2?

Yes. Cholecalciferol and ergocalciferol differ in mass and separate by HPLC. Results are reported in micrograms or IU against the label (1 microgram is 40 IU).

Can you test it in a finished product?

Yes. Capsules, powders, gummies and liquids are measured with sample preparation matched to the product, and reported in mg per serving or % w/w against the label.

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity against a reference standard. Full testing to the USP Cholecalciferol monograph adds the monograph's own tests and is quoted.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/5280795

Order

Send the product name, the form of the ingredient and the label claim for a quote, or submit a sample with the sample form.

Supplement testing