Biotin (vitamin B7)

Biotin: water-soluble vitamin (B7).

Biotin testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Biotin: 2D structure drawn from the PubChem record (CID 171548).

Compound data

CAS number

58-85-5

Formula

C₁₀H₁₆N₂O₃S

Molecular weight

244.31 g/mol

Type

Water-soluble vitamin (B7)

Structure and names

Identity: Biotin

IUPAC name: 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoic acid

How Vanguard tests Biotin

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monograph covers identity by infrared spectroscopy, optical rotation and HPLC retention time, assay by HPLC, and related compounds by HPLC. Full testing to the USP Biotin monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Biotin (CAS 58-85-5)
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Biotin testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC-UV for purity and quantity and LC-MS for identity. Full testing to the USP Biotin monograph is available and quoted.

Can biotin be measured in a finished gummy or capsule?

Yes. Biotin is often present at microgram levels per serving, so the sample is extracted and quantified against a reference standard by HPLC, with LC-MS confirming identity at low levels.

Does the test pick up oxidized biotin?

Biotin can oxidize to biotin sulfoxide and biotin sulfone. These elute as separate peaks in the HPLC purity profile and have different masses by LC-MS.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/171548

Identity facts from the USP monograph header for this compound.

Order

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