Colchicine

Colchicine: tropolone alkaloid.

Colchicine testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Colchicine: 2D structure drawn from the PubChem record (CID 6167).

Compound data

CAS number

64-86-8

Formula

C₂₂H₂₅NO₆

Molecular weight

399.44 g/mol

Type

Tropolone alkaloid

Structure and names

Identity: Colchicine

IUPAC name: N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[a]heptalen-7-yl]acetamide

How Vanguard tests Colchicine

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monograph covers identity by infrared spectroscopy, assay by HPLC, chromatographic purity by HPLC, a colchiceine limit by color test, ethyl acetate by GC, residual solvents, optical rotation and water content. Full testing to the USP Colchicine monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit
Microbiology (injectables)
Endotoxin	$210
Sterility or bioburden	$150
Container closure integrity (CCI)	$50
Screening
Fentanyl screen	$50

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Colchicine (CAS 64-86-8)
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Colchicine testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC with UV detection for purity and assay, and LC-MS for identity. Full testing to the USP Colchicine monograph is available and quoted.

What is colchiceine?

Colchiceine is a breakdown product formed when colchicine loses a methyl group. The monograph limits it separately, and it shows as its own peak in HPLC.

Is colchicine light sensitive?

Yes. Light converts colchicine into lumicolchicine isomers, so samples should stay in an amber or opaque container until shipping.

Why might ethyl acetate show up?

Colchicine can hold ethyl acetate from crystallization, which is why the monograph includes a GC limit for it.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/6167

Identity facts from the USP monograph header for this compound.

Order

Start an order online, or send the compound name and the tests you need for a quote.

All research compounds