Folic acid (vitamin B9)

Folic acid: water-soluble vitamin (B9).

Folic acid testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Folic acid: 2D structure drawn from the PubChem record (CID 135398658).

Compound data

CAS number

59-30-3

Formula

C₁₉H₁₉N₇O₆

Molecular weight

441.40 g/mol

Type

Water-soluble vitamin (B9)

Structure and names

Identity: Folic acid

IUPAC name: (2S)-2-[[4-[(2-amino-4-oxo-3H-pteridin-6-yl)methylamino]benzoyl]amino]pentanedioic acid

How Vanguard tests Folic acid

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monograph covers identity by UV spectroscopy, assay by HPLC, related compounds by HPLC, residue on ignition, and water content by titration. Full testing to the USP Folic Acid monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Folic Acid (CAS 59-30-3)
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Folic acid testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC-UV for purity and quantity and LC-MS for identity. Full testing to the USP Folic Acid monograph is available and quoted.

Is folic acid the same as methylfolate (5-MTHF)?

No. Folic acid and L-methylfolate are different compounds with different retention times and masses, so the test shows which one is present. A label claim for one cannot be confirmed by measuring the other.

Is folic acid sensitive to light?

Yes. Folic acid breaks down under light and heat to products such as p-aminobenzoylglutamic acid and pterin compounds, which show up as impurity peaks. Samples should be kept dark until testing.

Why does water content matter for folic acid?

Folic acid raw material holds a variable amount of water, and the USP assay is calculated on the anhydrous basis. An assay that ignores water can make good material look low.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/135398658

Identity facts from the USP monograph header for this compound.

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