Neem seed (Azadirachta indica)

Neem seed: botanical (seed and seed oil).

Neem seed testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

2D structure of a Neem seed marker compound

Marker compound: 2D structure drawn from the PubChem record (CID 108058).

Compound data

Botanical source

Azadirachta indica Seed

Marker compounds

nimbin, salannin, azadirachtin A

Basis

total or named markers, as your specification states

Type

Botanical (seed and seed oil)

Structure and names

Marker compounds: nimbin (CAS 5945-86-6), salannin (CAS 992-20-1), azadirachtin A (CAS 11141-17-6)

How Vanguard tests Neem seed

Method for the $250 identity, purity and quantity test: HPLC-UV marker-compound assay (nimbin and salannin), LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP Herbal Medicines Compendium monograph covers identity by HPTLC fingerprint and content of the limonoids nimbin and salannin by HPLC. Full testing to the USP Herbal Medicines Compendium monograph for Azadirachta indica Seed is available and quoted.

What's reported

  • Marker compounds, as % w/w on the basis your specification states
  • Quantity, against the label claim
  • Identity, reported as match or no match against the reference standard
  • With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on testPrice
Identity and impurities
FTIR identity$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)$125
Chiral purity (where a chiral method exists; new methods quoted)$250
Water, solvents and salt form
Water content by Karl Fischer$60
Residual solvents$150
Counterions: chloride, acetate and TFA$70
Assay
Assay, % w/w (with raw powder purity; $250 together)+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead$150
Conformity: purity and quantity on more units from the same lot$100 per extra unit

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

  • Azadirachta indica Seed
  • Azadirachta indica Seed Oil
  • Azadirachta indica Seed Powder
  • Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Neem seed testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC-UV measurement of nimbin and salannin, with LC-MS identity. Full testing to the USP Herbal Medicines Compendium monograph for Azadirachta indica Seed is available and quoted.

Is neem oil tested by GC or HPLC?

The limonoid markers nimbin and salannin are measured by HPLC. The oil's fatty acid profile is a separate GC test, quoted on request.

Is azadirachtin stable?

Azadirachtin breaks down with light, heat and moisture, and pressed oils usually contain less of it than the seed. Samples are stored cool and dark before analysis.

What is neem confused with?

The monograph lists Melia azedarach, chinaberry, as a potential confounding material. The limonoid profile helps tell the two apart.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/108058

Identity facts from the USP monograph header for this compound.

Order

Start an order online, or send the compound name and the tests you need for a quote.