Omega-3-acid ethyl esters (EPA and DHA ethyl esters)

Omega-3-acid ethyl esters: fatty acid ethyl esters (fish oil concentrate).

Omega-3-acid ethyl esters testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

2D structure of Omega-3-acid ethyl esters

Omega-3-acid ethyl esters: 2D structure drawn from the PubChem record (CID 9831415).

Compound data

CAS number

86227-47-6

Formula

C22H34O2

Molecular weight

330.51 g/mol

Type

Fatty acid ethyl esters (fish oil concentrate)

Structure and names

Identity: EPA ethyl ester (main component)

Marker compounds: EPA ethyl ester (CAS 86227-47-6), DHA ethyl ester (CAS 81926-94-5)

IUPAC name: ethyl (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoate

How Vanguard tests Omega-3-acid ethyl esters

Method for the $250 identity, purity and quantity test: Chromatographic assay, with detection selected for the compound, against a reference standard, with results in 3 to 5 business days.

The USP monograph covers identity by chromatographic retention time, assay of EPA, DHA and total omega-3 ethyl esters by chromatography, cholesterol by chromatography, oligomers by size-exclusion chromatography, dioxins, furans and PCBs, trace metals, UV absorbance, and acid, anisidine and peroxide values. Full testing to the USP Omega-3-Acid Ethyl Esters monograph is available and quoted.

What's reported

  • Purity, as percent of total peak area
  • Quantity, against the label claim
  • Identity, reported as match or no match against the reference standard
  • With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on testPrice
Identity and impurities
FTIR identity$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)$125
Chiral purity (where a chiral method exists; new methods quoted)$250
Water, solvents and salt form
Water content by Karl Fischer$60
Residual solvents$150
Counterions: chloride, acetate and TFA$70
Assay
Assay, % w/w (with raw powder purity; $250 together)+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead$150
Conformity: purity and quantity on more units from the same lot$100 per extra unit

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

  • Omega-3-Acid Ethyl Esters
  • Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Omega-3-acid ethyl esters testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by a chromatographic assay, with detection selected for the compound. Full testing to the USP Omega-3-Acid Ethyl Esters monograph is available and quoted.

Why is fish oil not tested by standard HPLC?

Fatty acid esters have little UV absorbance, so the lab uses a detection method suited to the compound. The test separates the individual fatty acids by chain length and number of double bonds.

Is an ethyl ester concentrate the same as a triglyceride fish oil?

No. Ethyl ester concentrates and triglyceride oils are different chemical forms. Triglyceride oils are converted to methyl esters before testing, so the form on the label decides the sample preparation.

Does oxidation affect the result?

Omega-3 oils oxidize readily. Peroxide and anisidine values measure that oxidation and are separate from the EPA and DHA assay.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/9831415

Identity facts from the USP monograph header for this compound.

Order

Start an order online, or send the compound name and the tests you need for a quote.