Phytonadione (vitamin K1)

Phytonadione: fat-soluble vitamin (K1).

Phytonadione testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Phytonadione: 2D structure drawn from the PubChem record (CID 4812).

Compound data

CAS number

81818-54-4

Formula

C₃₁H₄₆O₂

Molecular weight

450.70 g/mol

Type

Fat-soluble vitamin (K1)

Structure and names

Identity: Phytonadione

IUPAC name: 2-methyl-3-(3,7,11,15-tetramethylhexadec-2-enyl)naphthalene-1,4-dione

How Vanguard tests Phytonadione

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monograph covers identity by infrared and UV spectroscopy, assay by HPLC, Z-isomer content by HPLC, menadione by a colorimetric test, refractive index, and a neutrality check. Full testing to the USP Phytonadione monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Phytonadione (CAS 81818-54-4)
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Phytonadione testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC-UV for purity and quantity and LC-MS for identity. Full testing to the USP Phytonadione monograph is available and quoted.

What are the E and Z isomers of vitamin K1?

Phytonadione is mainly the E (trans) isomer with some Z (cis) isomer. HPLC separates the two, and the USP monograph sets a limit on Z content.

Can the test tell vitamin K1 from vitamin K2 (MK-7)?

Yes. Phylloquinone (K1) and menaquinone-7 have different side chains, masses and retention times.

Is vitamin K1 light sensitive?

Yes. It breaks down quickly in light, so samples should arrive in amber or foil-wrapped containers.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/4812

Identity facts from the USP monograph header for this compound.

Order

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