Vitamin A (retinol, retinyl acetate, retinyl palmitate)

Vitamin A: fat-soluble vitamin (A).

Vitamin A testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Vitamin A: 2D structure drawn from the PubChem record (CID 445354).

Compound data

CAS number

68-26-8

Formula

C₂₀H₃₀O

Molecular weight

286.45 g/mol

Type

Fat-soluble vitamin (A)

Structure and names

Identity: Retinol

IUPAC name: (2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraen-1-ol

How Vanguard tests Vitamin A

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monographs cover identity by a color reaction and TLC, assay of vitamin A by UV spectrophotometry or chromatography under the general vitamin A assay chapter, a UV absorbance ratio, and vitamin D by chromatography in the combination capsules. Full testing to the USP Vitamin A monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit
Microbiology (injectables)
Endotoxin	$210
Sterility or bioburden	$150
Container closure integrity (CCI)	$50
Screening
Fentanyl screen	$50

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Vitamin A
Oleovitamin A and D Capsules
Vitamin A Oral Liquid Preparation
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Vitamin A testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC-UV for purity and quantity and LC-MS for identity. Full testing to the USP Vitamin A monograph is available and quoted.

Which form of vitamin A is in my sample?

Supplements usually contain retinyl acetate or retinyl palmitate rather than free retinol. The esters separate by HPLC, and the result can be reported as the ester or as retinol.

Is vitamin A sensitive to light and air?

Yes. Retinol and its esters oxidize and isomerize with light, heat and air, so samples should be sealed, kept dark and shipped cool. Cis isomers can appear as separate peaks.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/445354

Identity facts from the USP monograph header for this compound.

Order

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