Acetylcholine chloride: quaternary ammonium choline ester.
Acetylcholine chloride testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.
Acetylcholine chloride: 2D structure drawn from the PubChem record (CID 6060).
60-31-1
C7H16ClNO2
181.66 g/mol
Quaternary ammonium choline ester
Identity: Acetylcholine chloride
IUPAC name: 2-acetyloxyethyl(trimethyl)azanium chloride
Method for the $250 identity, purity and quantity test: Chromatographic assay, with detection selected for the compound, against a reference standard, with results in 3 to 5 business days.
The USP monograph covers identity by infrared spectroscopy and a chloride test, assay by titration after alkaline hydrolysis, and melting point, acidity, loss on drying and residue on ignition. Full testing to the USP Acetylcholine Chloride monograph is available and quoted.
Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.
| Add-on test | Price |
|---|---|
| Identity and impurities | |
| FTIR identity | $90 |
| CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur) | $125 |
| Chiral purity (where a chiral method exists; new methods quoted) | $250 |
| Water, solvents and salt form | |
| Water content by Karl Fischer | $60 |
| Residual solvents | $150 |
| Counterions: chloride, acetate and TFA | $70 |
| Assay | |
| Assay, % w/w (with raw powder purity; $250 together) | +$50 |
| Metals and conformity | |
| Heavy metals: arsenic, cadmium, chromium, mercury and lead | $150 |
| Conformity: purity and quantity on more units from the same lot | $100 per extra unit |
| Microbiology (injectables) | |
| Endotoxin | $210 |
| Sterility or bioburden | $150 |
| Container closure integrity (CCI) | $50 |
| Screening | |
| Fentanyl screen | $50 |
10% off for 10 or more samples.
Need something else? Other tests are available on request.
Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.
No. The $250 test covers identity, purity and quantity by a chromatographic assay, with detection selected for the compound. Full testing to the USP Acetylcholine Chloride monograph is available and quoted.
It is very hygroscopic, and once it picks up water the ester slowly hydrolyzes to choline and acetic acid. A sample that has clumped or smells of vinegar has often started to degrade.
Both are white, water-soluble quaternary ammonium salts and look the same as powders. They separate cleanly in the test, since choline lacks the acetyl group.
Acetylcholine has no aromatic ring and almost no UV absorbance, so the lab uses a detection method suited to the compound.
https://pubchem.ncbi.nlm.nih.gov/compound/6060
Identity facts from the USP monograph header for this compound.
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