Ampicillin

Ampicillin: beta-lactam antibiotic (aminopenicillin).

Ampicillin testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

2D structure of Ampicillin

Ampicillin: 2D structure drawn from the PubChem record (CID 6249).

Compound data

CAS number

69-53-4

Formula

C16H19N3O4S

Molecular weight

349.41 g/mol

Type

Beta-lactam antibiotic (aminopenicillin)

Structure and names

Identity: Ampicillin (anhydrous)

IUPAC name: (2S,5R,6R)-6-[[(2R)-2-amino-2-phenylacetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid

How Vanguard tests Ampicillin

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monograph covers identity by infrared spectroscopy, assay by HPLC, related compounds by HPLC and a dimethylaniline limit by GC, and sterility, bacterial endotoxins, pH and water. Full testing to the USP Ampicillin monograph is available and quoted.

What's reported

  • Purity, as percent of total peak area
  • Quantity, against the label claim
  • Identity, reported as match or no match against the reference standard
  • With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on testPrice
Identity and impurities
FTIR identity$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)$125
Chiral purity (where a chiral method exists; new methods quoted)$250
Water, solvents and salt form
Water content by Karl Fischer$60
Residual solvents$150
Counterions: chloride, acetate and TFA$70
Assay
Assay, % w/w (with raw powder purity; $250 together)+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead$150
Conformity: purity and quantity on more units from the same lot$100 per extra unit
Microbiology (injectables)
Endotoxin$210
Sterility or bioburden$150
Container closure integrity (CCI)$50
Screening
Fentanyl screen$50

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

  • Ampicillin (CAS 69-53-4 anhydrous; CAS 7177-48-2 trihydrate)
  • Ampicillin Sodium
  • Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Ampicillin testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC with UV detection for purity and assay, and LC-MS for identity. Full testing to the USP Ampicillin monograph is available and quoted.

Does it matter whether ampicillin is anhydrous or the trihydrate?

Yes. The trihydrate is about 13 percent water by weight, so the same powder reads differently on an anhydrous basis. Results are reported on the basis you name.

What does ampicillin break down to?

The beta-lactam ring opens with water and heat to form penicilloic acids, and ampicillin can also form dimers and higher polymers. These show up as separate peaks on the HPLC purity run.

Is the sodium salt tested the same way?

The ampicillin part is measured the same way, but ampicillin sodium is more hygroscopic and less stable once exposed to moisture. Keep samples sealed and dry before shipping.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/6249

Identity facts from the USP monograph header for this compound.

Order

Start an order online, or send the compound name and the tests you need for a quote.