Belladonna: botanical (tropane alkaloid leaf).
Belladonna testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.
Marker compound: 2D structure drawn from the PubChem record (CID 174174).
Atropa belladonna Leaf
atropine (hyoscyamine, racemic), scopolamine
total or named markers, as your specification states
Botanical (tropane alkaloid leaf)
Marker compounds: atropine (hyoscyamine, racemic) (CAS 51-55-8), scopolamine (CAS 51-34-3)
Method for the $250 identity, purity and quantity test: HPLC-UV marker-compound assay (atropine, scopolamine), LC-MS identity, against a reference standard, with results in 3 to 5 business days.
The USP Belladonna Leaf and Belladonna Extract monographs cover identity by macroscopic and microscopic examination, content of atropine and scopolamine by gas chromatography, ash and pesticide residues, and a Herbal Medicines Compendium monograph for Atropa belladonna leaf is also listed. Full testing to the USP Belladonna Leaf monograph or the Herbal Medicines Compendium monograph for Atropa belladonna is available and quoted.
Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.
| Add-on test | Price |
|---|---|
| Identity and impurities | |
| FTIR identity | $90 |
| CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur) | $125 |
| Chiral purity (where a chiral method exists; new methods quoted) | $250 |
| Water, solvents and salt form | |
| Water content by Karl Fischer | $60 |
| Residual solvents | $150 |
| Counterions: chloride, acetate and TFA | $70 |
| Assay | |
| Assay, % w/w (with raw powder purity; $250 together) | +$50 |
| Metals and conformity | |
| Heavy metals: arsenic, cadmium, chromium, mercury and lead | $150 |
| Conformity: purity and quantity on more units from the same lot | $100 per extra unit |
| Microbiology (injectables) | |
| Endotoxin | $210 |
| Sterility or bioburden | $150 |
| Container closure integrity (CCI) | $50 |
| Screening | |
| Fentanyl screen | $50 |
10% off for 10 or more samples.
Need something else? Other tests are available on request.
Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.
No. The $250 test covers identity and marker content by HPLC with UV detection for atropine and scopolamine, and LC-MS for identity. Full testing to the USP Belladonna Leaf monograph or the Herbal Medicines Compendium monograph for Atropa belladonna is available and quoted.
They are the main tropane alkaloids in belladonna leaf, and the monographs define the material by its content of them. Hyoscyamine in the plant converts to atropine during extraction, so it is reported as atropine.
Henbane, datura and stramonium contain the same alkaloids in different ratios. The alkaloid ratio is a useful clue, but a full botanical identification needs the monograph's microscopic examination.
Dried leaf, powdered leaf and extracts can be tested. Tell us which form you are sending, since results are reported per gram of leaf or per gram of extract.
https://pubchem.ncbi.nlm.nih.gov/compound/174174
Identity facts from the USP monograph header for this compound.
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