Beta carotene

Beta carotene: carotenoid (provitamin A).

Beta carotene testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Beta carotene: 2D structure drawn from the PubChem record (CID 5280489).

Compound data

CAS number

7235-40-7

Formula

C₄₀H₅₆

Molecular weight

536.87 g/mol

Type

Carotenoid (provitamin A)

Structure and names

Identity: Beta carotene

IUPAC name: 1,3,3-trimethyl-2-[(1E,3E,5E,7E,9E,11E,13E,15E,17E)-3,7,12,16-tetramethyl-18-(2,6,6-trimethylcyclohexen-1-yl)octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohexene

How Vanguard tests Beta carotene

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monograph covers identity by UV-visible spectroscopy and HPLC, total carotenoids by UV-visible spectrophotometry, content of beta carotene and of alpha carotene and other related compounds by HPLC, residue on ignition, and loss on drying. Full testing to the USP Beta Carotene monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Beta Carotene (CAS 7235-40-7)
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Beta carotene testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC-UV for purity and quantity and LC-MS for identity. Full testing to the USP Beta Carotene monograph is available and quoted.

Does the test separate beta carotene from alpha carotene and cis isomers?

Yes. HPLC separates all-trans beta carotene from alpha carotene and cis isomers, which natural-source material often contains. A UV total carotenoid reading alone cannot tell them apart.

How should beta carotene samples be handled?

Beta carotene oxidizes quickly in light and air. Beadlets, oil suspensions and softgels should be kept sealed, cool and dark until testing.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/5280489

Identity facts from the USP monograph header for this compound.

Order

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