Calcifediol (25-hydroxyvitamin D3)

Calcifediol: vitamin D3 metabolite.

Calcifediol testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Calcifediol: 2D structure drawn from the PubChem record (CID 6441383).

Compound data

CAS number

63283-36-3

Formula

C₂₇H₄₄O₂ · H₂O

Molecular weight

418.65 g/mol

Type

Vitamin D3 metabolite

Structure and names

Identity: Calcifediol monohydrate

IUPAC name: (1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-1-[(2R)-6-hydroxy-6-methylheptan-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexan-1-ol;hydrate

How Vanguard tests Calcifediol

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monograph covers identity by infrared spectroscopy, assay by HPLC, and water content. Full testing to the USP Calcifediol monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Calcifediol (CAS 63283-36-3, monohydrate)
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Calcifediol testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC-UV for purity and quantity and LC-MS for identity. Full testing to the USP Calcifediol monograph is available and quoted.

Is calcifediol the same as vitamin D3?

No. Calcifediol is 25-hydroxyvitamin D3, which carries one more hydroxyl group than cholecalciferol and has a different mass, so LC-MS tells the two apart.

Why does the monohydrate matter?

Calcifediol is usually supplied as the monohydrate. The water adds to its molecular weight, so the assay is stated against the anhydrous or hydrate form as labeled.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/6441383

Identity facts from the USP monograph header for this compound.

Order

Start an order online, or send the compound name and the tests you need for a quote.

All research compounds