Calcitriol (1,25-dihydroxyvitamin D3)

Calcitriol: vitamin D3 metabolite.

Calcitriol testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Calcitriol: 2D structure drawn from the PubChem record (CID 5280453).

Compound data

CAS number

32222-06-3

Formula

C₂₇H₄₄O₃

Molecular weight

416.64 g/mol

Type

Vitamin D3 metabolite

Structure and names

Identity: Calcitriol (anhydrous)

IUPAC name: trans-(1R,3S,5Z)-5-[(2E)-2-[(1R,3aS,7aR)-1-[(2R)-6-hydroxy-6-methylheptan-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexane-1,3-diol

How Vanguard tests Calcitriol

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monograph covers identity by infrared spectroscopy and HPLC retention time, assay by HPLC, organic impurities by HPLC, and water content. Full testing to the USP Calcitriol monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Calcitriol (CAS 32222-06-3; monohydrate CAS 77326-95-5)
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Calcitriol testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC-UV for purity and quantity and LC-MS for identity. Full testing to the USP Calcitriol monograph is available and quoted.

Why is calcitriol hard to quantify in finished products?

Calcitriol is often present at microgram or lower levels in oils and capsules, so sensitive HPLC and LC-MS conditions are needed to measure it.

Is calcitriol sensitive to light and air?

Yes. It degrades with light, heat and oxygen and converts partly to pre-calcitriol in solution. Samples should arrive sealed, protected from light and cold.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/5280453

Identity facts from the USP monograph header for this compound.

Order

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