Cloxacillin (sodium and benzathine)

Cloxacillin: penicillin antibiotic (isoxazolyl penicillin).

Cloxacillin testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

2D structure of Cloxacillin

Cloxacillin: 2D structure drawn from the PubChem record (CID 6098).

Compound data

CAS number

61-72-3

Formula

C19H18ClN3O5S

Molecular weight

435.88 g/mol

Type

Penicillin antibiotic (isoxazolyl penicillin)

Structure and names

Identity: Cloxacillin

IUPAC name: (2S,5R,6R)-6-[[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid

How Vanguard tests Cloxacillin

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP Cloxacillin Sodium monograph covers identity by infrared spectroscopy, HPLC retention time and a sodium test, assay by HPLC, organic impurities by HPLC, a dimethylaniline limit, pH, water determination and sterility. Full testing to the USP Cloxacillin Benzathine monograph is available and quoted.

What's reported

  • Purity, as percent of total peak area
  • Quantity, against the label claim
  • Identity, reported as match or no match against the reference standard
  • With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on testPrice
Identity and impurities
FTIR identity$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)$125
Chiral purity (where a chiral method exists; new methods quoted)$250
Water, solvents and salt form
Water content by Karl Fischer$60
Residual solvents$150
Counterions: chloride, acetate and TFA$70
Assay
Assay, % w/w (with raw powder purity; $250 together)+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead$150
Conformity: purity and quantity on more units from the same lot$100 per extra unit
Microbiology (injectables)
Endotoxin$210
Sterility or bioburden$150
Container closure integrity (CCI)$50
Screening
Fentanyl screen$50

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

  • Cloxacillin Sodium (CAS 7081-44-9 monohydrate)
  • Cloxacillin Benzathine
  • Cloxacillin Benzathine Intramammary Infusion
  • Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Cloxacillin testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC-UV for purity and assay, with LC-MS to confirm identity. Full testing to the USP Cloxacillin Benzathine monograph is available and quoted.

Does it matter whether the sample is cloxacillin sodium or cloxacillin benzathine?

Yes. The two salts have very different molecular weights, so the same mass contains a different amount of cloxacillin. Tell us which salt is on the label and the assay is reported as cloxacillin or as the salt.

Is cloxacillin sensitive to moisture?

Yes. The beta-lactam ring opens with water and heat to form penicilloic acids, which show as separate peaks in the HPLC purity profile. Keep samples dry and sealed.

Can the intramammary infusion be tested?

Yes. It is an oily suspension, so cloxacillin is extracted from the base before HPLC, and the result is reported per syringe or against the label claim.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/6098

Identity facts from the USP monograph header for this compound.

Order

Start an order online, or send the compound name and the tests you need for a quote.