Ergocalciferol (vitamin D2)

Ergocalciferol: fat-soluble vitamin (D2).

Ergocalciferol testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Ergocalciferol: 2D structure drawn from the PubChem record (CID 5280793).

Compound data

CAS number

50-14-6

Formula

C₂₈H₄₄O

Molecular weight

396.65 g/mol

Type

Fat-soluble vitamin (D2)

Structure and names

Identity: Ergocalciferol

IUPAC name: (1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-1-[(E,2R,5R)-5,6-dimethylhept-3-en-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexan-1-ol

How Vanguard tests Ergocalciferol

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monograph covers identity by infrared and UV spectroscopy and HPLC retention time, assay by HPLC, reducing substances by a colorimetric test, melting range, and optical rotation. Full testing to the USP Ergocalciferol monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Ergocalciferol (CAS 50-14-6)
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Ergocalciferol testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC-UV for purity and quantity and LC-MS for identity. Full testing to the USP Ergocalciferol monograph is available and quoted.

Can the test tell vitamin D2 from vitamin D3?

Yes. Ergocalciferol (D2) and cholecalciferol (D3) differ in mass and separate by HPLC, so LC-MS confirms which one is present.

Why does previtamin D come up in vitamin D2 results?

In solution, vitamin D2 converts reversibly to pre-ergocalciferol with heat. The two are accounted for together so the result reflects the total vitamin D2.

Is ergocalciferol sensitive to light and air?

Yes. It degrades with light, air and heat, so samples should be sealed, kept dark and shipped cool.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/5280793

Identity facts from the USP monograph header for this compound.

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