Estradiol

Estradiol: estrogen (steroid hormone).

Estradiol testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Estradiol: 2D structure drawn from the PubChem record (CID 5757).

Compound data

CAS number

50-28-2

Formula

C₁₈H₂₄O₂

Molecular weight

272.38 g/mol

Type

Estrogen (steroid hormone)

Structure and names

Identity: Estradiol (anhydrous)

IUPAC name: (8R,9S,13S,14S,17S)-13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthrene-3,17-diol

How Vanguard tests Estradiol

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monograph covers identity by infrared and UV spectroscopy, assay by HPLC, chromatographic purity by HPLC, melting range, specific rotation and water content. Full testing to the USP Estradiol monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit
Microbiology (injectables)
Endotoxin	$210
Sterility or bioburden	$150
Container closure integrity (CCI)	$50
Screening
Fentanyl screen	$50

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Estradiol (CAS 50-28-2)
Estradiol Benzoate
Estradiol Cypionate
Estradiol Valerate
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Estradiol testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC with UV detection for purity and assay, and LC-MS for identity. Full testing to the USP Estradiol monograph is available and quoted.

Can you test estradiol esters such as valerate or cypionate?

Yes. Each ester is a different molecule with its own retention time and mass, so the report names the ester found. Quantity is reported for the ester itself, not converted to estradiol, unless you ask for that.

Estradiol is sometimes sold as a hemihydrate. Does that change the result?

It can. The hemihydrate carries a small amount of water, so the assay is lower on an as-is basis than on an anhydrous basis. Tell us which form is on the label.

Can the test tell estradiol from estrone or estriol?

Yes. The three estrogens separate by HPLC and differ in mass, so a mislabeled or mixed sample shows up in the purity and identity results.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/5757

Identity facts from the USP monograph header for this compound.

Order

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