Five-leaved chaste tree (Vitex negundo)

Five-leaved chaste tree: botanical (leaf).

Five-leaved chaste tree testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

2D structure of a Five-leaved chaste tree marker compound

Marker compound: 2D structure drawn from the PubChem record (CID 442416).

Compound data

Botanical source

Vitex negundo Leaf

Marker compounds

agnuside, negundoside

Basis

total or named markers, as your specification states

Type

Botanical (leaf)

Structure and names

Marker compounds: agnuside (CAS 11027-63-7), negundoside

How Vanguard tests Five-leaved chaste tree

Method for the $250 identity, purity and quantity test: HPLC-UV marker-compound assay (agnuside, negundoside), LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP Herbal Medicines Compendium monograph covers identity by HPTLC fingerprint and content of agnuside and negundoside by HPLC. Full testing to the USP Herbal Medicines Compendium monograph for Vitex negundo leaf is available and quoted.

What's reported

  • Marker compounds, as % w/w on the basis your specification states
  • Quantity, against the label claim
  • Identity, reported as match or no match against the reference standard
  • With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on testPrice
Identity and impurities
FTIR identity$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)$125
Chiral purity (where a chiral method exists; new methods quoted)$250
Water, solvents and salt form
Water content by Karl Fischer$60
Residual solvents$150
Counterions: chloride, acetate and TFA$70
Assay
Assay, % w/w (with raw powder purity; $250 together)+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead$150
Conformity: purity and quantity on more units from the same lot$100 per extra unit

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

  • Vitex negundo Leaf
  • Vitex negundo Leaf Dry Extract
  • Vitex negundo Leaf Powder
  • Vitex negundo Leaf Powder Extract
  • Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Five-leaved chaste tree testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC with UV detection for the marker compounds (agnuside and negundoside), and LC-MS for identity. Full testing to the USP Herbal Medicines Compendium monograph for Vitex negundo leaf is available and quoted.

What do agnuside and negundoside measure?

They are iridoid glycosides, and the monograph sets the leaf's quality as the sum of the two. A low total can mean old or poorly stored leaf, or a different plant.

Is Vitex negundo the same as chasteberry?

No. Chasteberry is the fruit of Vitex agnus-castus, a related species. Agnuside is found in both, but negundoside is characteristic of Vitex negundo, so the marker pattern helps tell them apart.

Which forms can be sent?

Dried leaf, leaf powder and leaf extracts all have their own monographs. Extracts are concentrated, so let us know the form so results are reported on the right basis.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/442416

Identity facts from the USP monograph header for this compound.

Order

Start an order online, or send the compound name and the tests you need for a quote.