Hydroxocobalamin (vitamin B12a)

Hydroxocobalamin: water-soluble vitamin (B12).

Hydroxocobalamin testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Hydroxocobalamin: 2D structure drawn from the PubChem record (CID 44475014).

Compound data

CAS number

13422-51-0

Formula

C₆₂H₈₉CoN₁₃O₁₅P

Molecular weight

1346.36 g/mol

Type

Water-soluble vitamin (B12)

Structure and names

Identity: Hydroxocobalamin

IUPAC name: cobalt(3+);[(2R,3S,4R,5S)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2R)-1-[3-[(1R,2R,3R,4Z,7S,9Z,12S,13S,14Z,17S,18S,19R)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2,7,12,17-tetrahydro-1H-corrin-21-id-3-yl]propanoylamino]propan-2-yl] phosphate;hydroxide

How Vanguard tests Hydroxocobalamin

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monograph covers identity by infrared spectroscopy, a cobalt test and HPLC retention time, assay by HPLC, related compounds by HPLC, residual solvents, pH, and water content. Full testing to the USP Hydroxocobalamin monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Hydroxocobalamin (CAS 13422-51-0)
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Hydroxocobalamin testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC-UV for purity and quantity and LC-MS for identity. Full testing to the USP Hydroxocobalamin monograph is available and quoted.

Can the test tell hydroxocobalamin from cyanocobalamin or methylcobalamin?

Yes. The cobalamins have different retention times and masses, so LC-MS confirms which form is present.

How should hydroxocobalamin samples be handled?

Cobalamins degrade in light, so samples should arrive in amber or foil-wrapped containers. Hydroxocobalamin is often supplied as the acetate or chloride salt, which changes the factor used to convert the assay result.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/44475014

Identity facts from the USP monograph header for this compound.

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