Methotrexate: antifolate (dihydrofolate reductase inhibitor).
Methotrexate testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.
Methotrexate: 2D structure drawn from the PubChem record (CID 126941).
59-05-2
C20H22N8O5
454.45 g/mol
Antifolate (dihydrofolate reductase inhibitor)
Identity: Methotrexate
IUPAC name: (2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid
Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.
The USP monograph covers identity by infrared and UV spectroscopy, assay by HPLC, related compounds and enantiomeric purity by HPLC, residue on ignition, and water content. Full testing to the USP Methotrexate monograph is available and quoted.
Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.
| Add-on test | Price |
|---|---|
| Identity and impurities | |
| FTIR identity | $90 |
| CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur) | $125 |
| Chiral purity (where a chiral method exists; new methods quoted) | $250 |
| Water, solvents and salt form | |
| Water content by Karl Fischer | $60 |
| Residual solvents | $150 |
| Counterions: chloride, acetate and TFA | $70 |
| Assay | |
| Assay, % w/w (with raw powder purity; $250 together) | +$50 |
| Metals and conformity | |
| Heavy metals: arsenic, cadmium, chromium, mercury and lead | $150 |
| Conformity: purity and quantity on more units from the same lot | $100 per extra unit |
| Microbiology (injectables) | |
| Endotoxin | $210 |
| Sterility or bioburden | $150 |
| Container closure integrity (CCI) | $50 |
| Screening | |
| Fentanyl screen | $50 |
10% off for 10 or more samples.
Need something else? Other tests are available on request.
Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.
No. The $250 test covers identity, purity and quantity by HPLC-UV for purity and quantity and LC-MS for identity. Full testing to the USP Methotrexate monograph is available and quoted.
No. Methotrexate is the L-form, and the D-enantiomer has the same mass and the same retention time on a standard column. Enantiomeric purity needs a chiral HPLC method, which is part of full USP monograph testing.
Yes. Methotrexate degrades under light to products that appear as extra peaks in the HPLC purity profile. Samples should be protected from light until testing.
Yes. The two are structurally related but differ in retention time and mass by LC-MS.
https://pubchem.ncbi.nlm.nih.gov/compound/126941
Identity facts from the USP monograph header for this compound.
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