Rifampin (rifampicin)

Rifampin: rifamycin antibiotic.

Rifampin testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Rifampin: 2D structure drawn from the PubChem record (CID 135398735).

Compound data

CAS number

13292-46-1

Formula

C₄₃H₅₈N₄O₁₂

Molecular weight

822.94 g/mol

Type

Rifamycin antibiotic

Structure and names

Identity: Rifampin

IUPAC name: [(7S,9E,11S,12R,13S,14R,15R,16R,17S,18S,19E,21Z)-2,15,17,27,29-pentahydroxy-11-methoxy-3,7,12,14,16,18,22-heptamethyl-26-[(E)-(4-methylpiperazin-1-yl)iminomethyl]-6,23-dioxo-8,30-dioxa-24-azatetracyclo[23.3.1.14,7.05,28]triaconta-1(29),2,4,9,19,21,25,27-octaen-13-yl] acetate

How Vanguard tests Rifampin

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monograph covers identity by infrared spectroscopy, assay by HPLC, related compounds by HPLC, pH, and loss on drying. Full testing to the USP Rifampin monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit
Microbiology (injectables)
Endotoxin	$210
Sterility or bioburden	$150
Container closure integrity (CCI)	$50
Screening
Fentanyl screen	$50

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Rifampin (CAS 13292-46-1)
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Rifampin testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC-UV for purity and quantity and LC-MS for identity. Full testing to the USP Rifampin monograph is available and quoted.

Is rifampin the same as rifampicin?

Yes. Rifampin is the US name and rifampicin is the international name for the same compound.

What degradation products are common in rifampin?

Rifampin oxidizes to rifampin quinone and hydrolyzes in acid to 3-formylrifamycin SV, and rifampin N-oxide is another known impurity. All are measured as related compounds in the HPLC profile.

Why is rifampin red?

Rifampin is a red to brown powder because of its extended conjugated ring system. That color means it absorbs well in the visible range, which helps HPLC detection.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/135398735

Identity facts from the USP monograph header for this compound.

Order

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