Sulfasalazine

Sulfasalazine: aminosalicylate (azo sulfonamide prodrug).

Sulfasalazine testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Sulfasalazine: 2D structure drawn from the PubChem record (CID 5339).

Compound data

CAS number

599-79-1

Formula

C₁₈H₁₄N₄O₅S

Molecular weight

398.39 g/mol

Type

Aminosalicylate (azo sulfonamide prodrug)

Structure and names

Identity: Sulfasalazine

IUPAC name: 2-hydroxy-5-[[4-(pyridin-2-ylsulfamoyl)phenyl]diazenyl]benzoic acid

How Vanguard tests Sulfasalazine

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monograph covers identity by infrared spectroscopy and visible absorption spectrum, assay by UV-visible spectrophotometry, related compounds by thin-layer chromatography, residue on ignition, chloride and sulfate limits, and loss on drying. Full testing to the USP Sulfasalazine monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit
Microbiology (injectables)
Endotoxin	$210
Sterility or bioburden	$150
Container closure integrity (CCI)	$50
Screening
Fentanyl screen	$50

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Sulfasalazine (CAS 599-79-1)
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Sulfasalazine testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC-UV for purity and quantity and LC-MS for identity. Full testing to the USP Sulfasalazine monograph is available and quoted.

Why does sulfasalazine look orange?

Sulfasalazine is an azo dye, joining sulfapyridine and mesalamine through an azo bond. The color is normal and its strong visible absorbance helps detection.

Can the test find free sulfapyridine or salicylic acid?

Yes. Sulfapyridine, salicylic acid and related azo compounds separate from sulfasalazine by HPLC and have different masses by LC-MS. The USP monograph checks these by thin-layer chromatography instead.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/5339

Identity facts from the USP monograph header for this compound.

Order

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