Thiamine (vitamin B1)

Thiamine: water-soluble vitamin (B1).

Thiamine testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Thiamine: 2D structure drawn from the PubChem record (CID 6202).

Compound data

CAS number

67-03-8

Formula

C₁₂H₁₈Cl₂N₄OS

Molecular weight

337.27 g/mol

Type

Water-soluble vitamin (B1)

Structure and names

Identity: Thiamine hydrochloride

IUPAC name: 2-[3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-1,3-thiazol-3-ium-5-yl]ethanol;chloride;hydrochloride

How Vanguard tests Thiamine

Method for the $250 identity, purity and quantity test: HPLC-UV purity and assay, LC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monographs cover identity by infrared spectroscopy and chemical tests (with a fluorescence test for the mononitrate), assay by HPLC, related compounds by HPLC, a nitrate or chloride limit test, residue on ignition, pH, and water or loss on drying. Full testing to the USP Thiamine Hydrochloride or Thiamine Mononitrate monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Thiamine Hydrochloride (CAS 67-03-8)
Thiamine Mononitrate (CAS 532-43-4)
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Thiamine testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by HPLC-UV for purity and quantity and LC-MS for identity. Full testing to the USP Thiamine Hydrochloride or Thiamine Mononitrate monograph is available and quoted.

Which thiamine salt is in my sample?

Supplements use either thiamine hydrochloride or thiamine mononitrate. Both give the same thiamine ion by LC-MS, so thiamine is quantified and the result is converted to the labeled salt using its molecular weight.

Is thiamine stable?

Thiamine breaks down with heat, in neutral to alkaline solutions and in the presence of sulfites. Degradation products appear as extra peaks in the HPLC purity profile.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/6202

Identity facts from the USP monograph header for this compound.

Order

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