Vitamin E (alpha tocopherol)

Vitamin E: fat-soluble vitamin (E).

Vitamin E testing at Vanguard Laboratory: identity, purity and quantity $250 per sample, with results in 3 to 5 business days.

Structure

Vitamin E: 2D structure drawn from the PubChem record (CID 2116).

Compound data

CAS number

10191-41-0

Formula

C₂₉H₅₀O₂

Molecular weight

430.71 g/mol

Type

Fat-soluble vitamin (E)

Structure and names

Identity: all-rac-alpha-tocopherol

IUPAC name: 2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-3,4-dihydrochromen-6-ol

How Vanguard tests Vitamin E

Method for the $250 identity, purity and quantity test: GC-FID assay and purity, GC-MS identity, against a reference standard, with results in 3 to 5 business days.

The USP monographs cover identity by a color reaction, optical rotation and GC retention time, assay of the tocopherol form by GC, solubility, and acidity or acid value by titration. Full testing to the USP Vitamin E monograph is available and quoted.

What's reported

Purity, as percent of total peak area
Quantity, against the label claim
Identity, reported as match or no match against the reference standard
With monograph testing (quoted): the monograph’s tests against its limits

Tests and prices

Purity and quantity

$250

Chromatographic purity and mg per unit by the method matched to the compound, identity against a reference standard. 1 unit.

Raw powder purity

$200

Chromatographic purity on bulk powder. 50 mg.

Add-on tests

Add-on test	Price
Identity and impurities
FTIR identity	$90
CHNS elemental analysis (carbon, hydrogen, nitrogen, sulfur)	$125
Chiral purity (where a chiral method exists; new methods quoted)	$250
Water, solvents and salt form
Water content by Karl Fischer	$60
Residual solvents	$150
Counterions: chloride, acetate and TFA	$70
Assay
Assay, % w/w (with raw powder purity; $250 together)	+$50
Metals and conformity
Heavy metals: arsenic, cadmium, chromium, mercury and lead	$150
Conformity: purity and quantity on more units from the same lot	$100 per extra unit

10% off for 10 or more samples.

Need something else? Other tests are available on request.

Sample amount and turnaround

Purity and quantity is $250 per sample (1 unit) and raw powder purity is $200 for 50 mg, with results in 3 to 5 business days. Send the material sealed and labeled, with the label claim and the form it contains.

Salts, forms and compendial references

Vitamin E Preparation
Vitamin E Polyethylene Glycol Succinate
Tell us the salt or form the label states, so quantity is reported on the same basis.

Questions about Vitamin E testing

Is the $250 test the same as USP monograph testing?

No. The $250 test covers identity, purity and quantity by GC-FID for purity and quantity and GC-MS for identity. Full testing to the USP Vitamin E monograph is available and quoted.

Why is vitamin E tested by GC instead of HPLC?

Tocopherols and their esters are oils that separate well by GC, which is also the technique the USP uses for vitamin E assay. GC-MS gives a clear mass spectrum for identity.

Can the test tell natural (d) from synthetic (dl) vitamin E?

Not by GC alone. RRR (d) and all-rac (dl) alpha tocopherol have the same mass and retention time, so telling them apart needs optical rotation or a chiral method.

Is tocopheryl acetate reported differently from tocopherol?

Yes. Tocopherol, tocopheryl acetate and tocopheryl succinate are different compounds with different molecular weights, so the result is reported for the form on the label.

Sources

https://pubchem.ncbi.nlm.nih.gov/compound/2116

Identity facts from the USP monograph header for this compound.

Order

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